验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
| Positive IHC detected in | Jurkat cells Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82707-13-RR targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 3, apoptosis-related cysteine peptidase |
| 别名 | CASP3, Caspase3, 230527B2, CASP 3, CASP-3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 17 kDa, 19 kDa |
| GenBank蛋白编号 | NM_032991 |
| 基因名称 | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_3670535 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P42574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Cleaved Caspase 3/P17/P19 antibody 82707-13-RR | Download protocol |
| WB protocol for Cleaved Caspase 3/P17/P19 antibody 82707-13-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Adv Res Discovery of pyrazole derivative IHMT-140 as a pancreatic cancer therapeutic agent via dual mechanisms DDR1 inhibition and methuosis induction. | ||
Adv Sci (Weinh) Glucose Deprivation-Induced Disulfidptosis via the SLC7A11-INF2 Axis: Pan-Cancer Prognostic Exploration and Therapeutic Validation | ||
Cell Death Differ TRIM21-driven K63-linked ubiquitination of RBM38c, as a novel interactor of BECN1, contributes to DNA damage-induced autophagy | ||
Cell Death Differ Targeting ADAR1 lactylation activates innate immune and overcomes chemoresistance in ovarian cancer. | ||
Cell Death Dis SIRT7-mediated NRF2 deacetylation promotes antioxidant response and protects against chemodrug-induced liver injury | ||
Cell Death Dis ENO1-mediated deoxycytidine synthesis and gemcitabine resistance by stabilizing RRM2 in pancreatic cancer. |
