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验证数据展示

  • WB analysis using 82284-1-RR

    WB analysis using 82284-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82284-1-RR

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT4 antibody included
  • IHC staining of mouse brain using 82284-1-RR

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPT4 antibody included
  • Affinity and Kinetic Characterization of 82284-1-RR

    Affinity and Kinetic Characterization of 82284-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

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SEPT4 Recombinant monoclonal antibody

SEPT4 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 82284-1-RR

产品说明书

CloneNo. 5O8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SEPT4 Ready-To-Use IHC Kit. SEPT4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82284-1-RR targets SEPT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3185

Product name: Recombinant human SEPT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-459 aa of BC018056

Sequence: NQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 septin 4
别名 hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION
计算分子量 459 aa, 53 kDa
观测分子量38-55 kDa
GenBank蛋白编号BC018056
基因名称 SEPT4
Gene ID (NCBI) 5414
RRIDAB_3086475
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43236
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

实验方案

Product Specific Protocols
WB protocol for SEPT4 antibody 82284-1-RRDownload protocol
IHC protocol for SEPT4 antibody 82284-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols