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验证数据展示

  • WB analysis using 81232-10-RR

    WB analysis using 81232-10-RR

    Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 81232-10-RR

    WB analysis using 81232-10-RR

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 81232-10-RR

    WB analysis using 81232-10-RR

    Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 81232-10-RR (Phospho-AKT (Thr308) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of HeLa using 81232-10-RR

    FC experiment of HeLa using 81232-10-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-AKT (Thr308) Recombinant antibody (81232-10-RR, Clone:242063B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-AKT (Thr308) Recombinant antibody

Phospho-AKT (Thr308) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81232-10-RR

产品说明书

CloneNo. 242063B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

AKT (Thr308), p-AKT, p-AKT (Thr308), p-AKT Thr308, p-AKT-Thr308

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HSC-T6 cells, Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81232-10-RR targets Phospho-AKT (Thr308) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT (Thr308), p-AKT, p-AKT (Thr308), p-AKT Thr308, p-AKT-Thr308
计算分子量 56 kDa
观测分子量60 kDa
GenBank蛋白编号BC000479
基因名称 AKT1
Gene ID (NCBI) 207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)

实验方案

Product Specific Protocols
WB protocol for Phospho-AKT (Thr308) antibody 81232-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols