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验证数据展示

  • WB analysis of human urine using 81078-6-RR

    WB analysis of human urine using 81078-6-RR

    human urine sample was subjected to SDS PAGE followed by western blot with 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 81078-6-RR

    IHC staining of human tonsillitis using 81078-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 81078-6-RR

    IHC staining of human tonsillitis using 81078-6-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81078-6-RR (HVEM/TNFRSF14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81078-6-RR

    Affinity and Kinetic Characterization of 81078-6-RR

    Biolayer interferometry (BLl) kinetic assays of 81078-6-RR against Human HVEM/TNFRSF14 were performed. The affinity constant is 3.00 nM.

HVEM/TNFRSF14 Recombinant monoclonal antibody

HVEM/TNFRSF14 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 81078-6-RR

产品说明书

CloneNo. 250728B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman urine
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81078-6-RR targets HVEM/TNFRSF14 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1275

Product name: recombinant human TNFRSF14 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 39-202 aa of BC002794

Sequence: LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
别名 TNFRSF14, ATAR, CD270, Herpes virus entry mediator A, Herpesvirus entry mediator A
计算分子量 30 kDa
观测分子量40 kDa
GenBank蛋白编号BC002794
基因名称 TNFRSF14
Gene ID (NCBI) 8764
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92956
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes.

实验方案

Product Specific Protocols
IHC protocol for HVEM/TNFRSF14 antibody 81078-6-RRDownload protocol
WB protocol for HVEM/TNFRSF14 antibody 81078-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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