Urine of six individual healthy human donors was measured. The human CD31 concentration of detected samples was determined to be 0.47 ng/mL with a range of 0.05 - 1.39 ng/mL.

THP-1 cells (3 x 10^6 cells/mL) were cultured in RPMI with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 1 ug/mL LPS for 1 day. Aliquots of the cell culture supernatants were removed, assayed for levels of CD31, measured 0.47 ng/mL and 0.74 ng/mL respectively.

Plasma of six individual healthy human donors was measured. The human CD31 concentration of detected samples was determined to be 18.66 ng/mL with a range of 9.29 - 51.94 ng/mL.

Sandwich ELISA standard curve of MP02437-2, Human CD31 Recombinant Matched Antibody Pair - PBS only. 80773-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4658. 80773-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL