Various lysates were subjected to SDS PAGE followed by western blot with 80613-2-RR (LATS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80613-2-PBS in a different storage buffer formulation.

The mean LATS1 concentration was determined to be 128.4 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 42.1 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 23.1 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load, 40.0 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 52.2 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load and 72.9 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load.

Sandwich ELISA standard curve of MP02921-1, Human LATS1 Recombinant Matched Antibody Pair - PBS only. 80613-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11140. 80613-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL.