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验证数据展示

  • WB analysis using 80323-1-RR

    WB analysis using 80323-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80323-1-RR (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 80323-1-RR

    WB analysis of HeLa using 80323-1-RR

    WB result of METTL3 antibody (80323-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

  • IHC staining of mouse testis using 80323-1-RR

    IHC staining of mouse testis using 80323-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included
  • IHC staining of human lung cancer using 80323-1-RR

    IHC staining of human lung cancer using 80323-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with METTL3 antibody included
  • IF Staining of mouse testis using 80323-1-RR

    IF Staining of mouse testis using 80323-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using METTL3 antibody (80323-1-RR, Clone: 2I9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 80323-1-RR

    IF Staining of Jurkat using 80323-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using METTL3 antibody (80323-1-RR, Clone: 2I9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80323-1-RR

    Affinity and Kinetic Characterization of 80323-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80323-1-RR against Human METTL3 were performed. The affinity constant is 44 nM.

  • Featured Product
  • KD/KO验证
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METTL3 Recombinant monoclonal antibody

METTL3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 80323-1-RR

产品说明书

CloneNo. 2I9
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse testis tissue, human colon cancer tissue, human oesophagus cancer tissue, human lung cancer tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inMouse testis tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80323-1-RR targets METTL3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原

CatNo: Ag7110

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 229-580 aa of BC001650

Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 methyltransferase like 3
别名 MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348
计算分子量 64 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC001650
基因名称 METTL3
Gene ID (NCBI) 56339
RRIDAB_2918887
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ86U44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for METTL3 antibody 80323-1-RRDownload protocol
IHC protocol for METTL3 antibody 80323-1-RRDownload protocol
IF protocol for METTL3 antibody 80323-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB,IF

Am J Pathol

METTL3-Driven m6A Modification of RBPJ Promotes Vascular Remodeling in Pulmonary Hypertension

Authors - Qiang Du
View Article