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验证数据展示

  • WB analysis of HEK-293 using 80294-2-RR

    WB analysis of HEK-293 using 80294-2-RR

    Non-treated HEK-293 cells and sodium arsenite treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 80294-2-RR

    WB analysis of NIH/3T3 using 80294-2-RR

    Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-P62/SQSTM1 (Ser349) Recombinant antibody

Phospho-P62/SQSTM1 (Ser349) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80294-2-RR

产品说明书

CloneNo. 250277E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

P62, P62/SQSTM1, SQSTM1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected insodium arsenite treated HEK-293 cells, MG132 treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80294-2-RR targets Phospho-P62/SQSTM1 (Ser349) in applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sequestosome 1
别名 P62, P62/SQSTM1, SQSTM1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
计算分子量 48 kDa
观测分子量 62 kDa
GenBank蛋白编号BC017222
基因名称 P62/SQSTM1
Gene ID (NCBI) 8878
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13501
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

实验方案

Product Specific Protocols
WB protocol for Phospho-P62/SQSTM1 (Ser349) antibody 80294-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols