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验证数据展示

  • WB analysis using 68878-1-Ig

    WB analysis using 68878-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68878-1-Ig (MDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

MDH2 Monoclonal antibody

MDH2 Monoclonal Antibody for WB, ELISA
Cat No. 68878-1-Ig

产品说明书

CloneNo. 2A9G11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA

别名

MDH 2, MDH, EC:1.1.1.37, 2A9G11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MDH2 Ready-To-Use IHC Kit. MDH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68878-1-Ig targets MDH2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7750

Product name: Recombinant human MDH2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-338 aa of BC001917

Sequence: MLSALARPVSAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 malate dehydrogenase 2, NAD (mitochondrial)
别名 MDH 2, MDH, EC:1.1.1.37, 2A9G11
计算分子量 36 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001917
基因名称 MDH2
Gene ID (NCBI) 4191
RRIDAB_3670445
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP40926
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).

实验方案

Product Specific Protocols
WB protocol for MDH2 antibody 68878-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols