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验证数据展示

  • WB analysis using 68554-1-Ig

    WB analysis using 68554-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68554-1-Ig (STARD10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 68554-1-Ig

    IP experiment of HepG2 using 68554-1-Ig

    IP result of anti-STARD10 (IP:68554-1-Ig, 4ug; Detection:68554-1-Ig 1:6000) with HepG2 cells lysate 1480 ug.

  • IF Staining of HeLa using 68554-1-Ig

    IF Staining of HeLa using 68554-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STARD10 antibody (68554-1-Ig, Clone: 1B9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

STARD10 Monoclonal antibody

STARD10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68554-1-Ig

产品说明书

CloneNo. 1B9A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, IP, ELISA

别名

Antigen NY CO 28, Antigen NY-CO-28, CGI 52, CGI-52, NY CO 28

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSK-BR-3 cells, MCF-7 cells, T-47D cells, LNCaP cells, rat liver tissue, mouse liver tissue, pig liver tissue, rabbit liver tissue, rabbit testis tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68554-1-Ig targets STARD10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag10932

Product name: Recombinant human STARD10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-291 aa of BC007919

Sequence: MEKLAASTEPQGPRPVLGRESVQVPDDQDFRSFRSECEAEVGWNLTYSRAGVSVWVQAVEMDRTLHKIKCRMECCDVPAETLYDVLHDIEYRKKWDSNVIETFDIARLTVNADVGYYSWRCPKPLKNRDVITLRSWLPMGADYIIMNYSVKHPKYPPRKDLVRAVSIQTGYLIQSTGPKSCVITYLAQVDPKGSLPKWVVNKSSQFLAPKAMKKMYKACLKYPEWKQKHLPHFKPWLHPEQSPLPSLALSELSVQHADSLENIDESAVAESREERMGGAGGEGSDDDTSLT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 StAR-related lipid transfer (START) domain containing 10
别名 Antigen NY CO 28, Antigen NY-CO-28, CGI 52, CGI-52, NY CO 28
计算分子量 291 aa, 33 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC007919
基因名称 STARD10
Gene ID (NCBI) 10809
RRIDAB_3085257
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

实验方案

Product Specific Protocols
WB protocol for STARD10 antibody 68554-1-IgDownload protocol
IF protocol for STARD10 antibody 68554-1-IgDownload protocol
IP protocol for STARD10 antibody 68554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols