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验证数据展示

  • WB analysis using 68552-1-Ig

    WB analysis using 68552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68552-1-Ig (RAB35 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

RAB35 Monoclonal antibody

RAB35 Monoclonal Antibody for WB, ELISA
Cat No. 68552-1-Ig

产品说明书

CloneNo. 3H3E1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat, rabbit

应用

WB, ELISA

别名

GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy RAB35 Ready-To-Use IHC Kit. RAB35 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68552-1-Ig targets RAB35 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原

CatNo: Ag21424

Product name: Recombinant human RAB35 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-201 aa of BC015931

Sequence: MARDYDHLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB35, member RAS oncogene family
别名 GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY
计算分子量 201 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC015931
基因名称 RAB35
Gene ID (NCBI) 11021
RRIDAB_3085256
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

实验方案

Product Specific Protocols
WB protocol for RAB35 antibody 68552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols