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验证数据展示

  • WB analysis using 68510-1-Ig (same clone as 68510-1-PBS)

    WB analysis using 68510-1-Ig (same clone as 68510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51782-1

    Cytometric bead array sample test of MP51782-1

    Cytometric bead array sample test of MP51782-1, CDCA8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68510-1-PBS. Detection antibody: 68510-3-PBS.

  • IF Staining of A431 using 68510-1-Ig (same clone as 68510-1-PBS)

    IF Staining of A431 using 68510-1-Ig (same clone as 68510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68510-1-Ig (same clone as 68510-1-PBS)

    IF Staining of A549 using 68510-1-Ig (same clone as 68510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51782-1

    Cytometric bead array standard curve of MP51782-1

    Cytometric bead array standard curve of MP51782-1, CDCA8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68510-1-PBS. Detection antibody: 68510-3-PBS. Standard:Ag3136. Range: 0.781-100 ng/mL.

CDCA8 Monoclonal antibody, PBS Only (Capture)

CDCA8 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68510-1-PBS

产品说明书

CloneNo. 2A10C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

2A10C9, BOR, Borealin, Cell division cycle-associated protein 8, Dasra B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDCA8 Ready-To-Use IHC Kit. CDCA8 primary antibody included.

产品信息

68510-1-PBS targets CDCA8 as part of a matched antibody pair:

MP51782-1: 68510-1-PBS capture and 68510-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3136

Product name: Recombinant human CDCA8 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-280 aa of BC016944

Sequence: MAPRKGSSRVANTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle associated 8
别名 2A10C9, BOR, Borealin, Cell division cycle-associated protein 8, Dasra B
计算分子量 280 aa, 31 kDa
观测分子量 31-38 kDa
GenBank蛋白编号BC016944
基因名称 CDCA8
Gene ID (NCBI) 55143
RRIDAB_3085219
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ53HL2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

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