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验证数据展示

  • WB analysis using 68346-1-Ig

    WB analysis using 68346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68346-1-Ig (ZMAT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ZMAT3 Monoclonal antibody

ZMAT3 Monoclonal Antibody for WB, ELISA
Cat No. 68346-1-Ig

产品说明书

CloneNo. 2D2E7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

FLJ12296, p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, U2OS cells, HepG2 cells, K-562 cells, hTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68346-1-Ig targets ZMAT3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag20552

Product name: Recombinant human ZMAT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-289 aa of BC002896

Sequence: MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPVPPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEAQSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPRSRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRNEMENLGYV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger, matrin type 3
别名 FLJ12296, p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3
计算分子量 32 kDa
观测分子量~32 kDa
GenBank蛋白编号BC002896
基因名称 ZMAT3
Gene ID (NCBI) 64393
RRIDAB_3085069
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9HA38
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMAT3 is a p53-induced zinc finger protein, and a role in the TP53-dependent growth regulatory pathway, contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Acts as a bona fide target gene of p53/TP53.

实验方案

Product Specific Protocols
WB protocol for ZMAT3 antibody 68346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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