• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 68342-1-Ig

    WB analysis using 68342-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68342-1-Ig (RABL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of A549 using 68342-1-Ig

    IF Staining of A549 using 68342-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABL3 antibody (68342-1-Ig, Clone: 2H3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RABL3 Monoclonal antibody

RABL3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68342-1-Ig

产品说明书

CloneNo. 2H3E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Rab like protein 3, RABL3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68342-1-Ig targets RABL3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33137

Product name: Recombinant human RABL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-236 aa of BC020832

Sequence: MASLDRVKVLVLGDSGVGKSSLVHLLCQNQVLGNPSWTVGCSVDVRVHDYKEGTPEEKTCYIELWDVGGSVGSASSVKSTRAVFYNSVNGIIFVHDLTNKKSSQNLRRWSLEALNRDLVPTGVLVTNGDYDQEQFADNQIPLLVIGTKLDQIHETKRHEVLTTTAFLAEDFNPEEINLDCTNPRYLAAGSSNAVKLSRFFDKVIEKRYFLREGNQIPGFPDRKRFGAGTLKSLHYD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB, member of RAS oncogene family-like 3
别名 Rab like protein 3, RABL3
计算分子量 236 aa, 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC020832
基因名称 RABL3
Gene ID (NCBI) 285282
RRIDAB_3085066
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ5HYI8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

实验方案

Product Specific Protocols
IF protocol for RABL3 antibody 68342-1-IgDownload protocol
WB protocol for RABL3 antibody 68342-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...