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验证数据展示

  • WB analysis using 68296-1-Ig

    WB analysis using 68296-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of mouse liver using 68296-1-Ig

    IF Staining of mouse liver using 68296-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 68296-1-Ig

    IF Staining of mouse liver using 68296-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7 antibody (68296-1-Ig, Clone: 3G2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 68296-1-Ig

    IHC staining of human pancreas using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human pancreas cancer using 68296-1-Ig

    IHC staining of human pancreas cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human renal cell carcinoma using 68296-1-Ig

    IHC staining of human renal cell carcinoma using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 68296-1-Ig

    IHC staining of human liver using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human kidney using 68296-1-Ig

    IHC staining of human kidney using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 68296-1-Ig

    IHC staining of human liver using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human pancreas cancer using 68296-1-Ig

    IHC staining of human pancreas cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human renal cell carcinoma using 68296-1-Ig

    IHC staining of human renal cell carcinoma using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 68296-1-Ig

    IHC staining of human lung cancer using 68296-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included

Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68296-1-Ig

产品说明书

CloneNo. 3G2H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

KRT7, 3G2H1, CK 7, CK7, CK-7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEC109 cells, HeLa cells, HaCat cells, A431 cells, A549 cells, BxPC-3 cells, T-47D cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4150-1:16600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68296-1-Ig targets Cytokeratin 7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7895

Product name: Recombinant human CK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC002700

Sequence: MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINRRTAAENEFVVLKKDVDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDAR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 7
别名 KRT7, 3G2H1, CK 7, CK7, CK-7
计算分子量 469 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC002700
基因名称 Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2935376
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP08729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 7 antibody 68296-1-IgDownload protocol
IHC protocol for Cytokeratin 7 antibody 68296-1-IgDownload protocol
WB protocol for Cytokeratin 7 antibody 68296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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