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验证数据展示

  • IF Staining of HepG2 using 68245-1-Ig

    IF Staining of HepG2 using 68245-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA2013 antibody (68245-1-Ig, Clone: 4G6C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68245-1-Ig

    WB analysis using 68245-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68245-1-Ig (KIAA2013 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68245-1-Ig

    WB analysis using 68245-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68245-1-Ig (KIAA2013 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

KIAA2013 Monoclonal antibody

KIAA2013 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68245-1-Ig

产品说明书

CloneNo. 4G6C4

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

4G6C4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68245-1-Ig targets KIAA2013 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 KIAA2013 fusion protein Ag27438 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA2013
别名 4G6C4
观测分子量65 kDa
GenBank蛋白编号BC035033
基因名称 KIAA2013
Gene ID (NCBI) 90231
RRIDAB_3085048
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA2013, also known as Q8IYS2 or MGC33867, is a single-pass transmembrane protein encoded by the KIAA2013 gene in humans. KIAA2013 has been found to play a role in the Endocannabinoid system. This system is made up of cannabinoid receptors 1 and 2 (CB1 and CB2) as well as the various ligands and enzymes that interact. The protein KIAA2013 has been found to be expressed within CB2 expressing cells. Both cannabinoid receptors are labeled as class A G-Protein Coupled Receptors, and CB2 is highly expressed within the human spleen and leukocytes. CB2, and by extension KIAA2013, are therefore targets of interest for therapeutic studies looking into diseases such as inflammatory bowel disease and rheumatoid arthritis(PMID:31616248 34752341).

实验方案

Product Specific Protocols
WB protocol for KIAA2013 antibody 68245-1-IgDownload protocol
IF protocol for KIAA2013 antibody 68245-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols