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验证数据展示

  • WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

    WB analysis using 68215-1-Ig (same clone as 68215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68215-1-Ig (RTN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve of MP50025-1

    Sandwich ELISA standard curve ofMP50025-1, RTN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68215-2-PBS. Detection antibody: HRP-conjugated 68215-1-PBS. Standard: Ag13766. Range: 0.625-20 ng/mL.

  • IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

    IP experiment of A549 using 68215-1-Ig (same clone as 68215-1-PBS)

    IP result of anti-RTN3 (IP:68215-1-Ig, 4ug; Detection:68215-1-Ig 1:2000) with A549 cells lysate 840 ug. This data was developed using the same antibody clone with 68215-1-PBS in a different storage buffer formulation.

RTN3 Monoclonal antibody, PBS Only (Detector)

RTN3 Monoclonal Antibody for WB, IP, Sandwich ELISA, Indirect ELISA
Cat No. 68215-1-PBS

产品说明书

CloneNo. 1E3H11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IP, Sandwich ELISA, Indirect ELISA

别名

ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68215-1-PBS targets RTN3 as part of a matched antibody pair:

MP50025-1: 68215-2-PBS capture and 68215-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag13766

Product name: Recombinant human RTN3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-236 aa of BC011394

Sequence: MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 reticulon 3
别名 ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1
计算分子量 113 kDa
观测分子量25-28 kDa
GenBank蛋白编号BC011394
基因名称 RTN3
Gene ID (NCBI) 10313
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO95197
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.