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验证数据展示

  • WB analysis using 68176-1-Ig

    WB analysis using 68176-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68176-1-Ig

    IF Staining of mouse brain using 68176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (68176-1-Ig, Clone: 2H2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of mouse retina using 68176-1-Ig

    WB analysis of mouse retina using 68176-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SYNGR1 Monoclonal antibody

SYNGR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68176-1-Ig

产品说明书

CloneNo. 2H2H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, ELISA

别名

Synaptogyrin-1, synaptogyrin 1, 2H2H1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse retina tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68176-1-Ig targets SYNGR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag31309

Product name: Recombinant human SYNGR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-92 aa of BC000731

Sequence: MEGGAYGAGKAGGAFDPYTLVRQPHTILRVVSWLFSIVVFGSIVNEGYLNSASEGEEFCIYNRNPNACSYGVAVGVLAFLTCLLYLALDVYF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptogyrin 1
别名 Synaptogyrin-1, synaptogyrin 1, 2H2H1
计算分子量 25 kDa
观测分子量29 kDa
GenBank蛋白编号BC000731
基因名称 SYNGR1
Gene ID (NCBI) 9145
RRIDAB_2935267
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43759
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

实验方案

Product Specific Protocols
IF protocol for SYNGR1 antibody 68176-1-IgDownload protocol
IHC protocol for SYNGR1 antibody 68176-1-IgDownload protocol
WB protocol for SYNGR1 antibody 68176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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