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验证数据展示

  • WB analysis using 68024-1-Ig

    WB analysis using 68024-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of MCF-7 using 68024-1-Ig

    WB analysis of MCF-7 using 68024-1-Ig

    WB result of SFXN1 antibody (68024-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells.

  • IF Staining of MCF-7 using 68024-1-Ig

    IF Staining of MCF-7 using 68024-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

SFXN1 Monoclonal antibody

SFXN1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68024-1-Ig

产品说明书

CloneNo. 2D9F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

2D9F1, sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, MCF-7 cells, LNCaP cells, HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, Rat brain tissue, Mouse brain tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68024-1-Ig targets SFXN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2985

Product name: Recombinant human SFXN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC020517

Sequence: MILIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAVVNYTNRSGDAPLTVNELGTAYVSATTGAVATALGLNALTKHVSPLIGRFVPFAAVAAANCINIPLMRQRELKVGIPVTDENGNRLGESANAAKQAITQVVVSRILMAAPGMAIPPFIMNTLEKKAFLKRFPWMSAPIQVGLVGFCLVFATPLCCALFPQKSSMSVTSLEAELQAKIQESHPELRRVYFNKGL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sideroflexin 1
别名 2D9F1, sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein
计算分子量35.6 kDa
观测分子量32 kDa
GenBank蛋白编号BC020517
基因名称 SFXN1
Gene ID (NCBI) 94081
RRIDAB_2918768
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H9B4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

实验方案

Product Specific Protocols
IF protocol for SFXN1 antibody 68024-1-IgDownload protocol
WB protocol for SFXN1 antibody 68024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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