验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, Jurkat cells |
| Positive IF/ICC detected in | LNCaP cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 1 publications below |
产品信息
67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IP |
| 经测试反应性 | Human |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag26995 Product name: Recombinant human PIK3CD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 395-580 aa of BC132919 Sequence: YAVIEKAKKARSTKKKSKKADCPIAWANLMLFDYKDQLKTGERCLYMWPSVPDEKGELLNPTGTVRSNPNTDSAAALLICLPEVAPHPVYYPALEKILELGRHSECVHVTEEEQLQLREILERRGSGELYEHEKDLVWKLRHEVQEHFPEALARLLLVTKWNKHEDVAQMLYLLCSWPELPVLSAL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, catalytic, delta polypeptide |
| 别名 | p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta |
| 计算分子量 | 1044 aa, 120 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC132919 |
| 基因名称 | PIK3CD |
| Gene ID (NCBI) | 5293 |
| RRID | AB_2918715 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00329 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PI3 Kinase p110 Delta antibody 67964-1-Ig | Download protocol |
| WB protocol for PI3 Kinase p110 Delta antibody 67964-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
