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验证数据展示

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51736-1

    Cytometric bead array sample test of MP51736-1

    Cytometric bead array sample test of MP51736-1, PXR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67912-1-PBS. Detection antibody: 67912-2-PBS.

  • Cytometric bead array sample test of MP51736-2

    Cytometric bead array sample test of MP51736-2

    Cytometric bead array sample test of MP51736-2, PXR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67912-1-PBS. Detection antibody: 67912-4-PBS.

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51736-1

    Cytometric bead array standard curve of MP51736-1

    Cytometric bead array standard curve of MP51736-1, PXR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67912-1-PBS. Detection antibody: 67912-2-PBS. Standard:Ag7991. Range: 3.125-400 ng/mL.

  • Cytometric bead array standard curve of MP51736-2

    Cytometric bead array standard curve of MP51736-2

    Cytometric bead array standard curve of MP51736-2, PXR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67912-1-PBS. Detection antibody: 67912-4-PBS. Standard:Ag7991. Range: 3.125-400 ng/mL.

PXR Monoclonal antibody, PBS Only (Capture)

PXR Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67912-1-PBS

产品说明书

CloneNo. 1G3A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

NR1I2, 1G3A7, BXR, ONR1, Orphan nuclear receptor PAR1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67912-1-PBS targets PXR as part of a matched antibody pair:

MP51736-1: 67912-1-PBS capture and 67912-2-PBS detection (validated in Cytometric bead array)

MP51736-2: 67912-1-PBS capture and 67912-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7991

Product name: Recombinant human PXR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 56-433 aa of BC017304

Sequence: MTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor subfamily 1, group I, member 2
别名 NR1I2, 1G3A7, BXR, ONR1, Orphan nuclear receptor PAR1
计算分子量 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC017304
基因名称 PXR
Gene ID (NCBI) 8856
RRIDAB_2918667
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75469
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].

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