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验证数据展示

  • WB analysis of human peripheral blood platelets using 67857-1-Ig

    WB analysis of human peripheral blood platelets using 67857-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 67857-1-Ig

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 67857-1-Ig

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67857-1-Ig

    IHC staining of human placenta using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD42b Monoclonal antibody

CD42b Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67857-1-Ig

产品说明书

CloneNo. 3G1F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhuman peripheral blood platelets, human blood
Positive IHC detected inmouse spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67857-1-Ig targets CD42b in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31181

Product name: Recombinant human GP1BA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 19-350 aa of BC027955

Sequence: ICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glycoprotein Ib (platelet), alpha polypeptide
别名 GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha
计算分子量 626 aa, 69 kDa
观测分子量145 kDa
GenBank蛋白编号BC027955
基因名称 CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_2918615
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07359
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

实验方案

Product Specific Protocols
IHC protocol for CD42b antibody 67857-1-IgDownload protocol
WB protocol for CD42b antibody 67857-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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