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验证数据展示

  • WB analysis using 67853-1-Ig

    WB analysis using 67853-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67853-1-Ig (DHX37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67853-1-Ig

    IF Staining of HeLa using 67853-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX37 antibody (67853-1-Ig, Clone: 2B12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 67853-1-Ig

    IF Staining of HeLa using 67853-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX37 antibody (67853-1-Ig, Clone: 2B12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

DHX37 Monoclonal antibody

DHX37 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67853-1-Ig

产品说明书

CloneNo. 2B12D9

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67853-1-Ig targets DHX37 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 DHX37 fusion protein Ag29909 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAH (Asp-Glu-Ala-His) box polypeptide 37
别名 DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517
计算分子量 1157 aa, 130 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC037964
基因名称 DHX37
Gene ID (NCBI) 57647
RRIDAB_2918611
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IY37
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

实验方案

Product Specific Protocols
WB protocol for DHX37 antibody 67853-1-IgDownload protocol
IF protocol for DHX37 antibody 67853-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols