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验证数据展示

  • WB analysis using 67779-1-Ig

    WB analysis using 67779-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67779-1-Ig (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67779-1-Ig

    IF Staining of HepG2 using 67779-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ABHD5 antibody (67779-1-Ig, Clone: 2B12C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ABHD5 Monoclonal antibody

ABHD5 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67779-1-Ig

产品说明书

CloneNo. 2B12C2

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

ABHD5, CDS, CGI58, IECN2, NCIE2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells, Raji cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ABHD5 Ready-To-Use IHC Kit. ABHD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67779-1-Ig targets ABHD5 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 ABHD5 fusion protein Ag30256 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 abhydrolase domain containing 5
别名 ABHD5, CDS, CGI58, IECN2, NCIE2
计算分子量 349 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC021958
基因名称 ABHD5
Gene ID (NCBI) 51099
RRIDAB_2918543
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WTS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

实验方案

Product Specific Protocols
WB protocol for ABHD5 antibody 67779-1-IgDownload protocol
IF protocol for ABHD5 antibody 67779-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols