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验证数据展示

  • WB analysis using 67730-1-Ig

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67730-1-Ig

    WB analysis of HEK-293 using 67730-1-Ig

    WB result of ZNF217 antibody (67730-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

  • IHC staining of mouse skin using 67730-1-Ig

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 67730-1-Ig

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ZNF217 Monoclonal antibody

ZNF217 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67730-1-Ig

产品说明书

CloneNo. 1F11B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ZFP217, 1F11B6, ZABC1, Zinc finger protein 217

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, 4T1 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, PC-3 cells, K-562 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67730-1-Ig targets ZNF217 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19468

Product name: Recombinant human ZNF217 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 707-1043 aa of BC113427

Sequence: IPSITCPFCTFKTFYPEVLMMHQRLEHKYNPDVHKNCRNKSLLRSRRTGCPPALLGKDVPPLSSFCKPKPKSAFPAQSKSLPSAKGKQSPPGPGKAPLTSGIDSSTLAPSNLKSHRPQQNVGVQGAATRQQQSEMFPKTSVSPAPDKTKRPETKLKPLPVAPSQPTLGSSNINGSIDYPAKNDSPWAPPGRDYFCNRSASNTAAEFGEPLPKRLKSSVVALDVDQPGANYRRGYDLPKYHMVRGITSLLPQDCVYPSQALPPKPRFLSSSEVDSPNVLTVQKPYGGSGPLYTCVPAGSPASSSTLEGKRPVSYQHLSNSMAQKRNYENFIGNAHYRP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger protein 217
别名 ZFP217, 1F11B6, ZABC1, Zinc finger protein 217
计算分子量 1048 aa, 115 kDa
观测分子量110-130 kDa
GenBank蛋白编号BC113427
基因名称 ZNF217
Gene ID (NCBI) 7764
RRIDAB_2882916
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF217 antibody 67730-1-IgDownload protocol
IHC protocol for ZNF217 antibody 67730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols