验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells, human testis tissue, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse liver tissue, rat pancreas tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat liver tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy RAB18 Ready-To-Use IHC Kit. RAB18 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:175-1:700 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67659-1-Ig targets RAB18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag16669 Product name: Recombinant human RAB18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-206 aa of BC015014 Sequence: MDEDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTISVDGNKAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEGLKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVKLSHREEGQGGGACGGYCSVL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAB18, member RAS oncogene family |
| 别名 | 2E4B3, RAB18LI1, Ras related protein Rab 18, Ras-related protein Rab-18 |
| 计算分子量 | 206 aa, 23 kDa |
| GenBank蛋白编号 | BC015014 |
| 基因名称 | RAB18 |
| Gene ID (NCBI) | 22931 |
| RRID | AB_2882856 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NP72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| IHC protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| WB protocol for RAB18 antibody 67659-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
