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验证数据展示

  • WB analysis using 67623-1-Ig

    WB analysis using 67623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • FC experiment of Jurkat using 67623-1-Ig

    FC experiment of Jurkat using 67623-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DIS3L2 Monoclonal antibody (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DIS3L2 Monoclonal antibody

DIS3L2 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67623-1-Ig

产品说明书

CloneNo. 2C9F2
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

hDIS3L2, FAM6A, EC:3.1.13.-, DIS3-like exonuclease 2, DIS3 like exonuclease 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DIS3L2 Ready-To-Use IHC Kit. DIS3L2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67623-1-Ig targets DIS3L2 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 DIS3L2 fusion protein Ag29992 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DIS3 mitotic control homolog (S. cerevisiae)-like 2
别名 hDIS3L2, FAM6A, EC:3.1.13.-, DIS3-like exonuclease 2, DIS3 like exonuclease 2
计算分子量 885 aa, 99 kDa
观测分子量 65 kDa, 99 kda
GenBank蛋白编号BC030113
基因名称 DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2882825
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IYB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

实验方案

Product Specific Protocols
WB protocol for DIS3L2 antibody 67623-1-IgDownload protocol
FC protocol for DIS3L2 antibody 67623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential

Authors - Damian M Janecki
View Article