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验证数据展示

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67534-1-Ig

    IF Staining of HepG2 using 67534-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67534-1-Ig (RALYL antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67534-1-Ig

    WB analysis using 67534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RALYL Monoclonal antibody

RALYL Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67534-1-Ig

产品说明书

CloneNo. 1F3F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

hRALYL, HNRPCL3, hnRNP core protein C-like 3, hnRNP core protein C like 3, Heterogeneous nuclear ribonucleoprotein C-like 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, LNCaP cells, IMR-32 cells, Daudi cells, NK-92 cells, HepG2 cells, Raji cells, rabbit brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67534-1-Ig targets RALYL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 RALYL fusion protein Ag11676 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RALY RNA binding protein-like
别名 hRALYL, HNRPCL3, hnRNP core protein C-like 3, hnRNP core protein C like 3, Heterogeneous nuclear ribonucleoprotein C-like 3
计算分子量 291 aa, 32 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC031090
基因名称 RALYL
Gene ID (NCBI) 138046
RRIDAB_2882753
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86SE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALYL antibody 67534-1-IgDownload protocol
IF protocol for RALYL antibody 67534-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols