验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67455-1-Ig targets CCT4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rabbit, Pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Mouse, Rabbit, Pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag17084 Product name: Recombinant human CCT4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 108-407 aa of BC106934 Sequence: SVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chaperonin containing TCP1, subunit 4 (delta) |
| 别名 | CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta |
| 计算分子量 | 539 aa, 58 kDa |
| 观测分子量 | 55-58 kDa |
| GenBank蛋白编号 | BC106934 |
| 基因名称 | CCT4 |
| Gene ID (NCBI) | 10575 |
| RRID | AB_2935250 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P50991 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCT4 antibody 67455-1-Ig | Download protocol |
| WB protocol for CCT4 antibody 67455-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
