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验证数据展示

  • WB analysis using 67392-1-Ig (same clone as 67392-1-PBS)

    WB analysis using 67392-1-Ig (same clone as 67392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    IF Staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51640-1

    Cytometric bead array sample test of MP51640-1

    Cytometric bead array sample test of MP51640-1, PPP4R1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67392-1-PBS. Detection antibody: 67392-2-PBS.

  • IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    IHC staining of human liver cancer using 67392-1-Ig (same clone as 67392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67392-1-Ig (same clone as 67392-1-PBS)

    FC experiment of HeLa using 67392-1-Ig (same clone as 67392-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67392-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51640-1

    Cytometric bead array standard curve of MP51640-1

    Cytometric bead array standard curve of MP51640-1, PPP4R1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67392-1-PBS. Detection antibody: 67392-2-PBS. Standard:Ag26750. Range: 0.781-100 ng/mL.

PPP4R1 Monoclonal antibody, PBS Only (Capture)

PPP4R1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 67392-1-PBS

产品说明书

CloneNo. 3D12G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

3D12G12, MEG1, PP4(Rmeg), PP4R1, Serine/threonine-protein phosphatase 4 regulatory subunit 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67392-1-PBS targets PPP4R1 as part of a matched antibody pair:

MP51640-1: 67392-1-PBS capture and 67392-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26750

Product name: Recombinant human PPP4R1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 331-520 aa of BC060829

Sequence: NRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRAS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 4, regulatory subunit 1
别名 3D12G12, MEG1, PP4(Rmeg), PP4R1, Serine/threonine-protein phosphatase 4 regulatory subunit 1
计算分子量 107 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC060829
基因名称 PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2882636
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8TF05
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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