验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
67273-1-Ig targets VANGL2 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | hamster |
| 免疫原 |
CatNo: Ag16980 Product name: Recombinant human VANGL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 268-322 aa of BC103920 Sequence: IQRVAVWILEKYYHDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vang-like 2 (van gogh, Drosophila) |
| 别名 | KIAA1215, Loop tail protein 1 homolog, LPP1, LTAP, STB1, STBM, STBM1, Strabismus 1, Van Gogh like protein 2, Vang like protein 2, VANGL2 |
| 计算分子量 | 521 aa, 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC103920 |
| 基因名称 | VANGL2 |
| Gene ID (NCBI) | 57216 |
| RRID | AB_2882542 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9ULK5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vangl2 is a key component of the planar cell polarity (PCP) pathway. Vangl2 is tightly associated with the postsynaptic density (PSD) fraction and forms a protein complex with PSD-95 and NMDA receptors. Vangl2 directly binds to the third PDZ domain of PSD-95 via its C-terminal TSV motif. Vangl2 directly binds to N-cadherin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VANGL2 antibody 67273-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
