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验证数据展示

  • WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

    WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51666-1

    Cytometric bead array sample test of MP51666-1

    Cytometric bead array sample test of MP51666-1, GCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67216-2-PBS. Detection antibody: 67216-1-PBS.

  • IF Staining of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)

    IF Staining of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCK antibody included
  • IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GCK antibody included
  • Cytometric bead array standard curve of MP51666-1

    Cytometric bead array standard curve of MP51666-1

    Cytometric bead array standard curve of MP51666-1, GCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67216-2-PBS. Detection antibody: 67216-1-PBS. Standard:Ag8116. Range: 3.125-400 ng/mL.

GCK Monoclonal antibody, PBS Only (Detector)

GCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67216-1-PBS

产品说明书

CloneNo. 1C3A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

1C3A3, EC:2.7.1.1, Glucokinase, Hexokinase-4, Hexokinase-D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GCK Ready-To-Use IHC Kit. GCK primary antibody included.

产品信息

67216-1-PBS targets GCK as part of a matched antibody pair:

MP51666-1: 67216-2-PBS capture and 67216-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag8116

Product name: Recombinant human GCK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 157-465 aa of BC001890

Sequence: EDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucokinase (hexokinase 4)
别名 1C3A3, EC:2.7.1.1, Glucokinase, Hexokinase-4, Hexokinase-D
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC001890
基因名称 GCK
Gene ID (NCBI) 2645
RRIDAB_2882508
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP35557
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

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