验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, pig heart tissue, pig skeletal muscle tissue, Rabbit skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:15000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67172-1-Ig targets FBXO32 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig, canine |
| 免疫原 |
CatNo: Ag25247 Product name: Recombinant human FBXO32 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-216 aa of BC024030 Sequence: IAQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETILHWQQQLNNIQITRPAFKGLTFTDLPLCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKKLCQYHFSERQIRKRLILSDKGQLDWKKMYFKLVRCYPRKEQYGDTLQLRKHCHILSWKGTDHPCTANNPESCSVSLSPQDFINLFKF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 32 |
| 别名 | 3C2G3, Atrogin 1, Atrogin-1, F box only protein 32, F box protein 32 |
| 计算分子量 | 355 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC024030 |
| 基因名称 | FBXO32 |
| Gene ID (NCBI) | 114907 |
| RRID | AB_2882468 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q969P5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXO32 antibody 67172-1-Ig | Download protocol |
| IHC protocol for FBXO32 antibody 67172-1-Ig | Download protocol |
| WB protocol for FBXO32 antibody 67172-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression | ||
Metabolism Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial | ||
Adv Sci (Weinh) Burn-Induced Gut Microbiota Dysbiosis Aggravates Skeletal Muscle Atrophy by Tryptophan-Kynurenine Mediated AHR Pathway Activation | ||
Cell Death Dis FBXO32-mediated degradation of PTEN promotes lung adenocarcinoma progression | ||
J Cachexia Sarcopenia Muscle Capsaicin alleviates cisplatin-induced muscle loss and atrophy in vitro and in vivo |
