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验证数据展示

  • WB analysis using 67136-1-Ig (same clone as 67136-1-PBS)

    WB analysis using 67136-1-Ig (same clone as 67136-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67136-1-Ig (same clone as 67136-1-PBS)

    IHC staining of human breast cancer using 67136-1-Ig (same clone as 67136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM21 antibody included
  • WB analysis of HeLa using 67136-1-Ig (same clone as 67136-1-PBS)

    WB analysis of HeLa using 67136-1-Ig (same clone as 67136-1-PBS)

    WB result of TRIM21 antibody (67136-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67136-1-Ig (same clone as 67136-1-PBS)

    IF Staining of HepG2 using 67136-1-Ig (same clone as 67136-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM21 antibody (67136-1-Ig, Clone: 1E8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50371-2

    Cytometric bead array standard curve of MP50371-2

    Cytometric bead array standard curve of MP50371-2, TRIM21 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67136-1-PBS. Detection antibody: 67136-3-PBS. Standard:Ag28377. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50371-4

    Cytometric bead array standard curve of MP50371-4

    Cytometric bead array standard curve of MP50371-4, TRIM21 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67136-1-PBS. Detection antibody: 67136-5-PBS. Standard:Ag28377. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

TRIM21 Monoclonal antibody, PBS Only (Capture)

TRIM21 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67136-1-PBS

产品说明书

CloneNo. 1E8B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1E8B9, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TRIM21 Ready-To-Use IHC Kit. TRIM21 primary antibody included.

产品信息

67136-1-PBS targets TRIM21 as part of a matched antibody pair:

MP50371-2: 67136-1-PBS capture and 67136-3-PBS detection (validated in Cytometric bead array)

MP50371-4: 67136-1-PBS capture and 67136-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28377

Product name: Recombinant human TRIM21 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 176-475 aa of BC010861

Sequence: SRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 21
别名 1E8B9, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, E3 ubiquitin-protein ligase TRIM21, EC:2.3.2.27, RING finger protein 81
计算分子量 475 aa, 54 kDa
观测分子量42-54 kDa
GenBank蛋白编号BC010861
基因名称 TRIM21
Gene ID (NCBI) 6737
RRIDAB_2882435
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP19474
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM21 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.

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