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验证数据展示

  • WB analysis of human brain using 67134-1-Ig

    WB analysis of human brain using 67134-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67134-1-Ig

    IHC staining of mouse brain using 67134-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67134-1-Ig

    IHC staining of mouse brain using 67134-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VILIP1 Monoclonal antibody

VILIP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67134-1-Ig

产品说明书

CloneNo. 2C6C3
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

VILIP 1, VILIP-1, VSNL1, 2C6C3, Hippocalcin like protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67134-1-Ig targets VILIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原

CatNo: Ag4923

Product name: Recombinant human VSNL1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-191 aa of BC022012

Sequence: MGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 visinin-like 1
别名 VILIP 1, VILIP-1, VSNL1, 2C6C3, Hippocalcin like protein 3
计算分子量 22 kDa
观测分子量 20 kDa
GenBank蛋白编号BC022012
基因名称 VSNL1
Gene ID (NCBI) 7447
RRIDAB_2882433
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP62760
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for VILIP1 antibody 67134-1-IgDownload protocol
WB protocol for VILIP1 antibody 67134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biomedicines

Characteristic Analysis of Featured Genes Associated with Cholangiocarcinoma Progression

Authors - Qigu Yao
View Article
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