验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, human heart tissue, pig heart tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67082-1-Ig targets Myogenin in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Rat, Pig |
| 文献引用反应性 | human, mouse, rabbit, bovine, goat |
| 免疫原 |
CatNo: Ag6414 Product name: Recombinant human Myogenin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC053899 Sequence: MELYETSPYFYQEPRFYDGENYLPVHLQGFEPPGYERTELTLSPEAPGPLEDKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myogenin (myogenic factor 4) |
| 别名 | bHLHc3, Myf 4, MYF4, MYOG, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4) |
| 计算分子量 | 25 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC053899 |
| 基因名称 | Myogenin |
| Gene ID (NCBI) | 4656 |
| RRID | AB_2882390 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15173 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Myogenin antibody 67082-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. | ||
Cell Mol Life Sci Ythdf2 facilitates precursor miR-378/miR-378-5p maturation to support myogenic differentiation | ||
Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. | ||
Int J Mol Sci G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle. | ||
Front Cell Dev Biol Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia. | ||
FASEB J Circular RNA circUSP13 sponges miR-29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1. |
