验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse stomach tissue, human placenta tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Planning an IHC experiment? We recommend our IHCeasy CNTROB Ready-To-Use IHC Kit. CNTROB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:175-1:700 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
67061-1-Ig targets CNTROB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25447 Product name: Recombinant human CNTROB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC021134 Sequence: MATSADSPSSPLGAEDLLSDSSEPPGLNQVSSEVTSQLYASLRLSRQAEATARAQLYLPSTSPPHEGLDGFAQELSRSLSVGLEKNLKKKDGSKHIFEMESVRGQLQTMLQTSRDTAYRDPLIPGAGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPLNPPDFQGLRDALDSEHTRRKHCERHIQSLQTRVLELQQQLAVAVAADRKKDTMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEEHQAAELTRSKQQETVTRLEQSLSEAMEALNREQESARLQQRERETLEEERQALTLRLEAEQQRCCVLQEERDAARAGQLSEHRELET 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | centrobin, centrosomal BRCA2 interacting protein |
| 别名 | 3D12A4, Centrobin, Centrosomal BRCA2-interacting protein, LIP8, LYST interacting protein 8 |
| 计算分子量 | 903 aa, 101 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC021134 |
| 基因名称 | CNTROB |
| Gene ID (NCBI) | 116840 |
| RRID | AB_2882371 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8N137 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| IHC protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| WB protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Glia Spatial Transcriptomic Analysis Reveals HDAC Inhibition Modulates Microglial Dynamics to Protect Against Ischemic Stroke in Mice | ||
Mol Biol Cell Binding of CEP152 to PLK4 stimulates kinase activity to promote centriole assembly |
