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验证数据展示

  • WB analysis using 67018-1-Ig (same clone as 67018-1-PBS)

    WB analysis using 67018-1-Ig (same clone as 67018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67018-1-Ig (STBD1 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STBD1 antibody included
  • IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STBD1 antibody included
  • IF Staining of HeLa using 67018-1-Ig (same clone as 67018-1-PBS)

    IF Staining of HeLa using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STBD1 antibody (67018-1-Ig, Clone: 1A2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50130-2

    Cytometric bead array standard curve of MP50130-2

    Cytometric bead array standard curve of MP50130-2, STBD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67018-1-PBS. Detection antibody: 67018-2-PBS. Standard:Ag28613. Range: 1.563-100 ng/mL.

STBD1 Monoclonal antibody, PBS Only (Capture)

STBD1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67018-1-PBS

产品说明书

CloneNo. 1A2G2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1A2G2, Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STBD1 Ready-To-Use IHC Kit. STBD1 primary antibody included.

产品信息

67018-1-PBS targets STBD1 as part of a matched antibody pair:

MP50130-2: 67018-1-PBS capture and 67018-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28613

Product name: Recombinant human STBD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-358 aa of BC022301

Sequence: GPGDTGKDGDAEQEKDAPLGGAAIPGGHQSGSSGLSPGPSGQELVTKPEHLQESNGHLISKTKDLGKLQAASWRLQNPSREVCDNSREHVPSGQFPDTEAPATSETSNSRSYSEVSRNESLESPMGEWGFQKGQEISAKAATCFAEKLPSSNLLKNRAKEEMSLSDLNSQDRVDHEEWEMVPRHSSWGDVGVGGSLKAPVLNLNQGMDNGRSTLVEARGQQVHGKMERVAVMPAGSQQVSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLETGHEDKVVHAWWGIH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 starch binding domain 1
别名 1A2G2, Genethonin-1, GENX-3414, Glycophagy cargo receptor STBD1, starch binding domain 1
计算分子量 41 kDa
观测分子量 38 kDa
GenBank蛋白编号BC022301
基因名称 STBD1
Gene ID (NCBI) 8987
RRIDAB_2882334
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95210
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

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