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验证数据展示

  • WB analysis of HUVEC using 66857-1-Ig

    WB analysis of HUVEC using 66857-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 66857-1-Ig

    IHC staining of human lymphoma using 66857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 66857-1-Ig

    IHC staining of human lymphoma using 66857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACVRL1 Monoclonal antibody

ACVRL1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66857-1-Ig

产品说明书

CloneNo. 2E11B10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, Daudi cells, human placenta tissue, JAR cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66857-1-Ig targets ACVRL1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 ACVRL1 fusion protein Ag6446 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 activin A receptor type II-like 1
别名 Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I
计算分子量 56 kDa
观测分子量 56 kDa, 63 kDa
GenBank蛋白编号BC042637
基因名称 ACVRL1
Gene ID (NCBI) 94
RRIDAB_2882196
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37023
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

实验方案

Product Specific Protocols
WB protocol for ACVRL1 antibody 66857-1-IgDownload protocol
IHC protocol for ACVRL1 antibody 66857-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols