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  • KD/KO Validated

Cytokeratin 5 Monoclonal antibody

Cytokeratin 5 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66727-1-Ig

产品说明书

CloneNo. 1A1C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig and More (1)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

CK 5, CK-5, keratin 5, KRT5, 1A1C5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHaCaT cells, MCF-7 cells, A549 cells, mouse skin tissue, SKOV-3 cells, A431 cells, A-253 cells, human saliva, pig skin tissue
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human breast hyperplasia tissue, human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman oesophagus cancer tissue, human prostate cancer tissue, human lung cancer tissue, mouse eye tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66727-1-Ig targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, pig
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag24184

Product name: Recombinant human KRT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 316-491 aa of BC024292

Sequence: THVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 5
别名 CK 5, CK-5, keratin 5, KRT5, 1A1C5
计算分子量 590 aa, 62 kDa
观测分子量 60 kDa
GenBank蛋白编号BC024292
基因名称 Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2882077
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13647
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
IHC protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
WB protocol for Cytokeratin 5 antibody 66727-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer

Integrated single-cell and spatial transcriptomic profiling decodes lineage plasticity and immune microenvironment remodeling in prostate cancer progression.

Authors - Hongyuan Yu
humanIHC,IF

Nat Genet

Spatially resolved transcriptomic analysis of the adult human prostate

Authors - Junyi Hu
mouseIF

Nat Commun

UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment

Authors - Yin Li
humanIF

Metabolism

Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19

Authors - Tian-Zi Wen
mouseIF

Biomark Res

Single-cell analysis reveals alternations between the aged and young mice prostates

Authors - Yang Li
humanIHC

Cell Death Dis

SERPINB3 enhances NPM1 sumoylation via inhibiting SENP3's activity and promotes lung tumorigenesis.

Authors - Xiangyu Meng
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