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验证数据展示

  • WB analysis of rat brain using 66709-1-Ig

    WB analysis of rat brain using 66709-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66709-1-Ig (MAG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66709-1-Ig

    IHC staining of mouse brain using 66709-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66709-1-Ig (MAG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAG Monoclonal antibody

MAG Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66709-1-Ig

产品说明书

CloneNo. 3H9G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66709-1-Ig targets MAG in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag6065

Product name: Recombinant human MAG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 199-516 aa of BC053347

Sequence: VSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myelin associated glycoprotein
别名 GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A
计算分子量 69 kDa
观测分子量 100 kDa
GenBank蛋白编号BC053347
基因名称 MAG
Gene ID (NCBI) 4099
RRIDAB_2882061
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP20916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 66709-1-IgDownload protocol
IHC protocol for MAG antibody 66709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols