验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CBL Ready-To-Use IHC Kit. CBL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | c-Cbl fusion protein Ag23033 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| 别名 | C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55 |
| 计算分子量 | 906 aa, 100 kDa |
| 观测分子量 | 100-120 kDa |
| GenBank蛋白编号 | BC132733 |
| 基因名称 | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_2881936 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22681 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| IHC protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.
| ||
