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验证数据展示

  • WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    IP experiment of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    IP result of anti-FOXO3A (IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    IHC staining of human breast cancer using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXO3A antibody included
  • IF Staining of HepG2 using 66428-1-Ig (same clone as 66428-1-PBS)

    IF Staining of HepG2 using 66428-1-Ig (same clone as 66428-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG(H+L). This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    WB analysis of HEK-293 using 66428-1-Ig (same clone as 66428-1-PBS)

    WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    WB analysis using 66428-1-Ig (same clone as 66428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66428-1-PBS in a different storage buffer formulation.

  • Sample test of MP51341-1

    Sample test of MP51341-1

    Sample test of MP51341-1, FOXO3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66428-1-PBS. Detection antibody: 66428-2-PBS.

  • Cytometric bead array standard curve of MP51341-1

    Cytometric bead array standard curve of MP51341-1

    Cytometric bead array standard curve of MP51341-1, FOXO3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66428-1-PBS. Detection antibody: 66428-2-PBS. Standard:Ag1289. Range: 0.391-400 ng/mL.

  • Featured Product
  • KD/KO验证

FOXO3A Monoclonal antibody, PBS Only (Capture)

FOXO3A Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 66428-1-PBS

产品说明书

CloneNo. 1F12D11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test

别名

FOXO3, 1F12D11, AF6q21, AF6q21 protein, FKHRL1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FOXO3A Ready-To-Use IHC Kit. FOXO3A primary antibody included.

产品信息

66428-1-PBS targets FOXO3A as part of a matched antibody pair:

MP51341-1: 66428-1-PBS capture and 66428-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 FOXO3A fusion protein Ag1289 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box O3
别名 FOXO3, 1F12D11, AF6q21, AF6q21 protein, FKHRL1
计算分子量 673 aa, 71 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC021224
基因名称 FOXO3A
Gene ID (NCBI) 2309
RRIDAB_2881799
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43524
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)