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验证数据展示

  • IHC staining of human lung cancer using 66333-1-Ig

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66333-1-Ig

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66333-1-Ig

    WB analysis using 66333-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NR8383 using 66333-1-Ig

    WB analysis of NR8383 using 66333-1-Ig

    NR8383 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IGSF2/CD101 Monoclonal antibody

IGSF2/CD101 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66333-1-Ig

产品说明书

CloneNo. 1E6A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, NR8383 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66333-1-Ig targets IGSF2/CD101 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 IGSF2/CD101 fusion protein Ag23297 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin superfamily, member 2
别名 IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101
观测分子量 120 kDa
GenBank蛋白编号BC136484
基因名称 IGSF2
Gene ID (NCBI) 9398
RRIDAB_2881713
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93033
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.

实验方案

Product Specific Protocols
WB protocol for IGSF2/CD101 antibody 66333-1-IgDownload protocol
IHC protocol for IGSF2/CD101 antibody 66333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols