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验证数据展示

  • WB analysis of HeLa using 66244-1-Ig

    WB analysis of HeLa using 66244-1-Ig

    WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

  • WB analysis using 66244-1-Ig

    WB analysis using 66244-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human breast hyperplasia using 66244-1-Ig

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 66244-1-Ig

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CNN2 Monoclonal antibody

CNN2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66244-1-Ig

产品说明书

CloneNo. 1G10F5

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

1G10F5, calponin 2, calponin2, Calponin-2, CNN 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, pig stomach tissue, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66244-1-Ig targets CNN2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag23594

Product name: Recombinant human CNN2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 224-309 aa of BC141818

Sequence: PGTRRHIYDTKLGTDKCDNSSMSLQMGYTQGANQSGQVFGLGRQIYDPKYCPQGTVADGAPSGTGDCPDPGEVPEYPPYYQEEAGY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calponin 2
别名 1G10F5, calponin 2, calponin2, Calponin-2, CNN 2
计算分子量 309 aa, 34 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC141818
基因名称 CNN2
Gene ID (NCBI) 1265
RRIDAB_2881633
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99439
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

实验方案

Product Specific Protocols
IHC protocol for CNN2 antibody 66244-1-IgDownload protocol
WB protocol for CNN2 antibody 66244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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