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验证数据展示

  • IHC staining of human breast cancer using 66237-1-Ig

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mammaglobin A antibody included
  • IHC staining of human breast cancer using 66237-1-Ig

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mammaglobin A antibody included
  • IF Staining of human breast cancer using 66237-1-Ig

    IF Staining of human breast cancer using 66237-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Mammaglobin A antibody (66237-1-Ig, Clone: 2E5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mammaglobin A Monoclonal antibody

Mammaglobin A Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66237-1-Ig

产品说明书

CloneNo. 2E5D4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF-P, ELISA

别名

SCGB2A2, 2E5D4, hMAM, Mammaglobin 1, Mammaglobin1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy Mammaglobin A Ready-To-Use IHC Kit. Mammaglobin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66237-1-Ig targets Mammaglobin A in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22485

Product name: Recombinant human SCGB2A2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-93 aa of BC128252

Sequence: MKLLMVLMLAALSQHCYAGSGCPLLENVISKTINPQVSKTEYKELLQEFIDDNATTNAIDELKECFLNQTDETLSNVEVFMQLIYDSSLCDLF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 secretoglobin, family 2A, member 2
别名 SCGB2A2, 2E5D4, hMAM, Mammaglobin 1, Mammaglobin1
计算分子量 93 aa, 10 kDa
GenBank蛋白编号BC128252
基因名称 Mammaglobin A
Gene ID (NCBI) 4250
RRIDAB_2881626
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammaglobin A, also known as SCGB2A2, is a member of the secretoglobin superfamily. Mammaglobin A is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. This monoclonal antibody is raised against full-length of 93-amino acid human mammaglobin A.

实验方案

Product Specific Protocols
IF protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
IHC protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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