验证数据展示

WB analysis of HEK-293 using 66219-1-Ig
WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.
WB analysis using 66219-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 66219-1-Ig
Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).
IHC staining of mouse heart using 66219-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with N-cadherin antibody included
IHC staining of mouse heart using 66219-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with N-cadherin antibody included
IF Staining of mouse heart using 66219-1-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 66219-1-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66219-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with N-cadherin antibody included
IHC staining of human ovary tumor using 66219-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with N-cadherin antibody included
Affinity and Kinetic Characterization of 66219-1-Ig
Biolayer interferometry (BLl) kinetic assays of 66219-1-PBS against Human N-cadherin were performed. The affinity constant is 12.9 nM.

Featured Product
KD/KO验证

N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66219-1-Ig

产品说明书

CloneNo. 1D8B3

引用文献(218)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (218)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	rabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells
Positive IHC detected in	mouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue

Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:7500-1:30000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 184 publications below
IHC	See 22 publications below
IF	See 32 publications below

产品信息

66219-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用	WB, IHC, IF-P, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat, pig, rabbit
文献引用反应性	human, mouse, rat, pig, monkey
免疫原	CatNo: Ag4996 Product name: Recombinant human N-cadherin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 33-350 aa of BC036470 Sequence: KTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ 种属同源性预测
宿主/亚型	Mouse / IgG1
抗体类别	Monoclonal
产品类型	Antibody
全称	cadherin 2, type 1, N-cadherin (neuronal)
别名	1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2
计算分子量	906 aa, 100 kDa
观测分子量	130 kDa
GenBank蛋白编号	BC036470
基因名称	N-cadherin
Gene ID (NCBI)	1000
RRID	AB_2881610
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein G purification
UNIPROT ID	P19022
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

实验方案

Product Specific Protocols
IF protocol for N-cadherin antibody 66219-1-Ig	Download protocol
IHC protocol for N-cadherin antibody 66219-1-Ig	Download protocol
WB protocol for N-cadherin antibody 66219-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
monkey	IF	Cell Stem Cell Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques Authors - Yongshun Lin View Article
human	WB	Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. Authors - Jiangfeng Li View Article
human	WB	J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics Authors - Ye Young Shin View Article
human	WB,IHC	EBioMedicine PMID: 31594754 Authors - Yiyi Liu View Article
human	WB	Aging Cell Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation. Authors - Liling Xiong View Article
human	IF	Mater Today Bio Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening. Authors - Xinglong Zhu View Article

验证数据展示

WB analysis of HEK-293 using 66219-1-Ig

WB analysis using 66219-1-Ig

WB analysis of PC-3 using 66219-1-Ig

IHC staining of mouse heart using 66219-1-Ig

IHC staining of mouse heart using 66219-1-Ig

IF Staining of mouse heart using 66219-1-Ig

IF Staining of mouse heart using 66219-1-Ig

IHC staining of human ovary tumor using 66219-1-Ig

IHC staining of human ovary tumor using 66219-1-Ig

Affinity and Kinetic Characterization of 66219-1-Ig

N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

PMID: 31594754

Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.

Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening.