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验证数据展示

  • WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

    WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

    WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51440-1

    Cytometric bead array sample test of MP51440-1

    Cytometric bead array sample test of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS.

  • Cytometric bead array standard curve of MP51440-1

    Cytometric bead array standard curve of MP51440-1

    Cytometric bead array standard curve of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS. Standard:Ag7575. Range: 1.563-200 ng/mL.

  • Featured Product
  • KD/KO验证

USP5 Monoclonal antibody, PBS Only (Capture)

USP5 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66213-1-PBS

产品说明书

CloneNo. 3F12C10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

3F12C10, Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66213-1-PBS targets USP5 as part of a matched antibody pair:

MP51440-1: 66213-1-PBS capture and 66213-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7575

Product name: Recombinant human USP5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC005139

Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 5 (isopeptidase T)
别名 3F12C10, Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT
计算分子量 96 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC005139
基因名称 USP5
Gene ID (NCBI) 8078
RRIDAB_2881604
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP45974
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

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