验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66191-1-Ig targets VAPB in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, monkey, yeast |
| 免疫原 |
CatNo: Ag6313 Product name: Recombinant human VAPB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 31-180 aa of BC001712 Sequence: KLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | VAMP (vesicle-associated membrane protein)-associated protein B and C |
| 别名 | 4F6A6, ALS8, UNQ484/PRO983, VAMP associated protein B/C, VAMP B |
| 计算分子量 | 27 kDa |
| 观测分子量 | 30-32 kDa |
| GenBank蛋白编号 | BC001712 |
| 基因名称 | VAPB |
| Gene ID (NCBI) | 9217 |
| RRID | AB_2881586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O95292 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for VAPB antibody 66191-1-Ig | Download protocol |
| IHC protocol for VAPB antibody 66191-1-Ig | Download protocol |
| WB protocol for VAPB antibody 66191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites
| ||
Autophagy The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease | ||
Nat Commun ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress | ||
Nat Commun RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether. | ||
Nat Commun Restructured membrane contacts rewire organelles for human cytomegalovirus infection |
