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验证数据展示

  • WB analysis of human brain using 66106-1-Ig

    WB analysis of human brain using 66106-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66106-1-Ig (SULT4A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66106-1-Ig

    IHC staining of mouse brain using 66106-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66106-1-Ig (SULT4A1 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66106-1-Ig

    IHC staining of mouse brain using 66106-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66106-1-Ig (SULT4A1 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 66106-1-Ig

    IF Staining of mouse brain using 66106-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SULT4A1 antibody (66106-1-Ig, Clone: 1B9F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 66106-1-Ig

    IF Staining of mouse brain using 66106-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SULT4A1 antibody (66106-1-Ig, Clone: 1B9F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SULT4A1 Monoclonal antibody

SULT4A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66106-1-Ig

产品说明书

CloneNo. 1B9F6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

1B9F6, Brain sulfotransferase-like protein, EC:2.8.2.-, hBR-STL, hBR-STL-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHuman brain, tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66106-1-Ig targets SULT4A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17962

Product name: Recombinant human SULT4A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-284 aa of BC022459

Sequence: MAESEAETPSTPGEFESKYFEFHGVRLPPFCRGKMEEIANFPVRPSDVWIVTYPKSGTSLLQEVVYLVSQGADPDEIGLMNIDEQLPVLEYPQPGLDIIKELTSPRLIKSHLPYRFLPSDLHNGDSKVIYMARNPKDLVVSYYQFHRSLRTMSYRGTFQEFCRRFMNDKLGYGSWFEHVQEFWEHRMDSNVLFLKYEDMHRDLVTMVEQLARFLGVSCDKAQLEALTEHCHQLVDQCCSAEALPVGRGRVGLWKDIFTVSMNEKFDLVYKQKMGKCDLTFDFYL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sulfotransferase family 4A, member 1
别名 1B9F6, Brain sulfotransferase-like protein, EC:2.8.2.-, hBR-STL, hBR-STL-1
计算分子量 284 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC022459
基因名称 SULT4A1
Gene ID (NCBI) 25830
RRIDAB_2881505
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BR01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT4A1, also named as SULTX3, NST, ST4A1, hBR-STL and hBR-STL-1, belongs to the sulfotransferase 1 family. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. SULT4A1 displays activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphtylamine, and 2-naphtol. It may have a role in the sulfation of drugs and neurotransmitters in the CNS. SULT4A1 is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It is a novel cytosolic sulfotransferase that is primarily expressed in the brain.

实验方案

Product Specific Protocols
WB protocol for SULT4A1 antibody 66106-1-IgDownload protocol
IHC protocol for SULT4A1 antibody 66106-1-IgDownload protocol
IF protocol for SULT4A1 antibody 66106-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols